Urine is an excretory substance of human body. Though a healthy person’s urine contains no significant microbes
still if bacteria or yeast are introduced through the urinary tract then they can develop symptoms of UTI (Urinary
Tract Infection). Urine, a culture medium allows microbes to grow rapidly in room temperature. Presently, UTIs are
common and top listed nosocomial infection, and poses threat for both patients & other personnel in hospital. We
conducted a cross-sectional study on 186 patients (male 71 and female 115) who were admitted to different wards of
CMOSH from December-January 2017-18. We collected mid-stream urine specimens from the patient in an aseptic
way which were then cultured on McConkey, Blood and HiChrome UTI media. The specimens that showed ≥10^5
cfu/ml were regarded as positive for bacterial infection and antimicrobial susceptibility tests (Kirby-Bauer disk
diffusion method) were performed on those isolates. Among the 91 culture-confirmed positive UTIs (29.6 % male
and 60.9% female), 21 and 70 UTIs were due to gram positive and gram negative bacteria respectively. We found
the responsible pathogens to be E coli (51.6%), Klebsiella pneumoniae (22%), Staphylococcus aureus (13.2%),
Proteus mirabilis (5.5%), Enterococcus spp. (4.4%) and Pseudomonas aeruginosa (3.3%). Awareness on UTI,
recognition of its causative factors, prevention, and treatment- all these fours should be emphasized highly for the
sake of public health.